尼日利亚药用植物Euphorbia lateriflora和Ficus thonningii的叶圈中有一种特定的微生物群-动脉网 (2024)

AbstractThe microbiota of medicinal plants is known to be highly specific and can contribute to medicinal activity. However, the majority of plant species have not yet been studied. Here, we investigated the phyllosphere composition of two common Nigerian medicinal plants, Euphorbia lateriflora and Ficus thonningii, by a polyphasic approach combining analyses of metagenomic DNA and isolates.

摘要已知药用植物的微生物群具有高度特异性,可以促进药物活性。然而,大多数植物物种尚未被研究。。

Microbial abundance estimated via qPCR using specific marker gene primers showed that all leaf samples were densely colonized, with up to 108 per gram of leaf, with higher bacterial and fungal abundance than Archaea. While no statistically significant differences between both plant species were found for abundance, amplicon sequencing of 16S rRNA and ITS genes revealed distinct microbiota compositions.

使用特异性标记基因引物通过qPCR估计的微生物丰度表明,所有叶片样品都被密集定植,每克叶片高达108个,细菌和真菌丰度高于古细菌。。

Only seven of the 27 genera isolated were represented on both plants, e.g. dominant Sphingomonas spp., and numerous members of Xanthomonadaceae and Enterobacteriaceae. The most dominant fungal families on both plants were Cladosporiaceae, Mycosphaerellaceae and Trichosphaeriaceae. In addition, 225 plant-specific isolates were identified, with Pseudomonadota and Enterobacteriaceae being dominant.

分离的27个属中只有7个在两种植物上都有代表性,例如优势鞘氨醇单胞菌属,以及黄单胞菌科和肠杆菌科的许多成员。两种植物上最主要的真菌家族是枝孢菌科,分枝杆菌科和毛孢菌科。此外,鉴定出225种植物特异性分离株,其中假单胞菌和肠杆菌科占优势。

Interestingly, 29 isolates are likely species previously unknown, and 14 of these belong to Burkholderiales. However, a high proportion, 56% and 40% of the isolates from E. lateriflora and F. thonningii, respectively, were characterized as various Escherichia coli. The growth of most of the bacterial isolates was not influenced by extractable secondary metabolites of plants.

有趣的是,29个分离株可能是以前未知的物种,其中14个属于伯克霍尔德氏菌。然而,来自E.lateriflora和F.thonningii的分离株中分别有56%和40%的高比例被表征为各种大肠杆菌。大多数细菌分离株的生长不受植物可提取次级代谢产物的影响。

Our results suggest that a specific and diverse microbial community inhabits the leaves of both E. lateriflora and F. thonningii, including potentially new species and producers of antimicrobials..

我们的研究结果表明,一个特定且多样的微生物群落栖息在E.lateriflora和F.thonningii的叶片中,包括潜在的新物种和抗菌剂生产者。。

IntroductionThe phyllosphere refers to the shoots of plants, including leaves, stems, fruits and flowers1,2. As with almost every other environmental niche of plants, the phyllosphere is colonized by microorganisms, with bacteria being the most dominant1,2,3,4. Microorganisms coexisting with multicellular organisms confer a wide range of benefits1, including protection from pathogens and stress, nutrient modulation, synthesis of secondary metabolites and growth promotion.

引言叶圈是指植物的芽,包括叶,茎,果实和花1,2。与几乎所有其他植物的环境生态位一样,叶际被微生物定殖,细菌是最主要的1,2,3,4。与多细胞生物共存的微生物具有广泛的益处1,包括免受病原体和压力的保护,营养调节,次级代谢产物的合成和生长促进。

For many host organisms, the microbiome in each niche is shaped by antimicrobial principles produced by the host. While this was shown for the rhizosphere5,6, less is known about the phyllosphere.A great proportion of research on medicinal plants has focused on their antimicrobial properties, leading to the discovery of compounds with antibacterial and other biological activities, but the microbiome is almost totally neglected in such bioprospecting initiatives7,8,9,10.

对于许多宿主生物,每个生态位中的微生物组都是由宿主产生的抗菌原理塑造的。虽然这显示了根际5,6,但对叶际的了解较少。对药用植物的大部分研究都集中在它们的抗菌特性上,导致发现具有抗菌和其他生物活性的化合物,但在这种生物勘探活动中,微生物组几乎完全被忽视7,8,9,10。

Medicinal plants should be seen as meta-organisms consisting of the plants themselves and the microbes they harbour and efforts are thus being made to investigate the microbiology of medicinal plants’ phyllospheres. This interest is aroused partly because some bacteria coexisting with medicinal plants contribute to the production of secondary metabolites and active compounds5,11,12, and play roles in the metabolism and ecology of the plant.

药用植物应被视为由植物本身及其所含微生物组成的元生物,因此正在努力研究药用植物叶际的微生物学。引起这种兴趣的部分原因是,一些与药用植物共存的细菌有助于产生次级代谢产物和活性化合物5,11,12,并在植物的代谢和生态中发挥作用。

Indeed, some studies have confirmed that some plant-associated microbes themselves directly produce antimicrobial compounds, while others are involved in the production of secondary metabolites by such plants13,14,15,16,17,18. It is also possible that the microbiomes themselves are shaped by bioactive agents produced by the plants6.

事实上,一些研究已经证实,一些与植物相关的微生物本身直接产生抗菌化合物,而另一些则参与这些植物产生次级代谢产物13,14,15,16,17,18。微生物组本身也可能由植物产生的生物活性剂形成6。

Phyllosphere studies are increasingly featured in the literature, bu.

叶圈研究越来越多地出现在文献中。

Data availability

数据可用性

The assembled 16S rRNA gene sequences generated from cultured isolates are available under an NCBI Bioproject with accession numbers OQ396403-OQ396569. Sequences from the targeted metagenomics (fungal ITS and bacterial 16S rRNA genes) are available under the NCBI SRA database BioSample accessions; SAMN38151107 and SAMN38151108 for Euphorbia lateriflora and Ficus thonningii respectively.

从培养的分离株产生的组装的16S rRNA基因序列可在NCBI生物项目下获得,登录号为OQ396403-OQ396569。;SAMN38151107和SAMN38151108分别用于大戟和榕树。

https://www.ncbi.nlm.nih.gov/biosample/38151107, https://www.ncbi.nlm.nih.gov/biosample/38151108..

https://www.ncbi.nlm.nih.gov/biosample/38151107-是的,https://www.ncbi.nlm.nih.gov/biosample/38151108..

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Download referencesAcknowledgementsWe thank Dr Jonathan Wilson for part of the development of the bacterial isolation protocol. AOA is an Ernst Mach Fellow of the Austrian Agency for Education and Internationalization. INO is a Calestous Juma Science Leadership Fellow supported by the Bill and Melinda Gates Foundation (INV-036234).FundingThis work was part-supported through an International Development Fund Award from the UK Microbiology Society (IDF15/02), which provided resources for an inquiry-based laboratory course that trained and started AOO in this research.

下载参考文献致谢我们感谢乔纳森·威尔逊博士为细菌分离方案的部分开发。AOA是奥地利教育和国际化机构的Ernst Mach研究员。伊诺是比尔和梅琳达·盖茨基金会(INV-036234)支持的Calestous Juma Science Leadership研究员。资助这项工作得到了英国微生物学会(IDF15/02)国际发展基金奖的部分支持,该奖项为一项基于探究的实验室课程提供了资源,该课程在这项研究中培训并启动了AOO。

The UK Microbiology Society had no influence in the design and all other phases of the study.Author informationAuthors and AffiliationsDepartment of Pharmaceutical Microbiology, University of Ibadan, Ibadan, NigeriaAnderson O. Oaikhena,Morenike E. Coker,Dorothy Cyril-Okoh&Iruka N. OkekeDepartment of Environmental Biotechnology, Graz University of Technology, Graz, AustriaAnderson O.

。作者信息作者和附属机构Ibadan大学药物微生物学系,Ibadan,NigeriaAnderson O.Oaikhena,Morenike E.Coker,Dorothy Cyril Okoh&Iruka N.OkekeDepartment of Environmental Biotechnology,格拉茨理工大学,格拉茨,澳大利亚安德森O。

Oaikhena,Wisnu A. Wicaksono,Expedito Olimi&Gabriele BergAuthorsAnderson O. OaikhenaView author publicationsYou can also search for this author in.

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PubMedGoogle ScholarContributionsAOO, MEC and INO conceptualized and designed the study. AOO, DC, OE and WAW prepared materials, collected data and performed data analyses. AOO prepared the first draft and revisions. MEC, GB and INO critically reviewed the article. WAW, MEC, GB and INO supervised the study.

PubMed谷歌学术贡献SAOO,MEC和INO概念化并设计了这项研究。AOO、DC、OE和WAW准备材料,收集数据并进行数据分析。AOO准备了初稿和修订版。MEC,GB和INO批判性地审查了这篇文章。WAW,MEC,GB和INO监督了这项研究。

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Reprints and permissionsAbout this articleCite this articleOaikhena, A.O., Coker, M.E., Cyril-Okoh, D. et al. The phyllosphere of Nigerian medicinal plants, Euphorbia lateriflora and Ficus thonningii is inhabited by a specific microbiota.

转载和许可本文引用本文Oaikhena,A.O.,Coker,M.E.,Cyril Okoh,D。等人。尼日利亚药用植物大戟(Euphorbia lateriflora)和榕树(Ficus thonningii)的叶面居住着一种特定的微生物群。

Sci Rep 14, 22806 (2024). https://doi.org/10.1038/s41598-024-68001-wDownload citationReceived: 22 November 2023Accepted: 18 July 2024Published: 01 October 2024DOI: https://doi.org/10.1038/s41598-024-68001-wShare this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

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KeywordsPhyllosphere

关键词鞘氨醇

Euphorbia lateriflora

大戟

Ficus thonningii

通尼尼榕

Medicinal plantsAntimicrobials

药用植物抗菌剂

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尼日利亚药用植物Euphorbia lateriflora和Ficus thonningii的叶圈中有一种特定的微生物群-动脉网 (2024)
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